Details-enriched characterization of mobile populations. Morphological information from photos can include to your richness of flow cytometry details. By way of example, the figure displays an otherwise traditional apoptosis assay utilizing Annexin V and PI, adding cell imaging to characterize cells in Just about every population to expose morphologically distinct features. These insights couldn't have already been gained from multiplex staining on your own.
As samples are acquired around the Attune CytPix Flow Cytometer, the high-speed brightfield digicam captures and merchants visuals of detected events at a level approximately six,000 illustrations or photos for every second, depending on the flow level and graphic measurement. For increased versatility, Attune Cytometric Software package permits you to regulate the picture seize frequency as necessary and choose a certain gate for image seize, if required.
(B) Uncooked 264.7 macrophages in many cases are annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The number of singlets obtainable for Examination boosts once the user trains and employs a more correct product.
Find new insights into your experimental data by visualizing populations using combined fluorescence and morphology details. Our software program interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment which have been crafted in the software for an intuitive, streamlined facts Evaluation practical experience.
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Listed here, a highly trained consumer has gated singlets confidently. Following assessing the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of more than 4% aggregates.
Optimizing gating technique. Even robust manual singlet gating is mistake-vulnerable and remains a subjective choice place in Pretty much all flow cytometry assays. Imaging can be used to substantiate and change gates to incorporate only solitary cells of fascination.
Our scientists will style and design panels for yourself as many times you'll need—cost-free, no product or service obtain essential.
The Attune NxT cytometer here could be the trustworthy workhorse instrument your lab can rely on for steady and trustworthy general performance. The Attune CytPix design includes a large-velocity brightfield digital camera and AI-driven graphic Examination program, with new upgrades like device Understanding equipment and an intuitive consumer interface, enabling Improved facts precision, precision, and quicker insights into sample biology.
Buyers can perspective function pictures in true-time to verify sample high quality, enhance protocols, and report the morphology of each and every cell inhabitants determined for Investigation. Imaging seize also benefits from acoustic concentrating, which helps you to posture the cells so that a sharp, centered picture is received.
AI-driven automated graphic analysis Together with the Attune Cytometric Application translates event characteristics into distinct morphology parameters that may be combined with conventional fluorescence and scatter parameters to expose really reproducible populace variations and unique subsets.
We Earlier shown the strength of imaging Car or truck-T/Ramos mobile interactions. Allow’s check out just the population of biggest fascination, the double favourable gatherings, To find out more. We will now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to even more study the features of such populations and refine gating on these occasions, expanding information robustness.
Leverage the power of advanced technological innovation and innovation to accelerate your discoveries. Have confidence in from the Attune Flow Cytometer spouse and children to provide the dependability, speed, and precision that established your function aside.
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Gating on these extended parameters enables you to swiftly and accurately establish populations of curiosity and confirm gating system with little if any handbook overview. The Attune application provides use of point out-of-the-artwork device Mastering analysis equipment inside an expertly created person interface, rendering it extremely easy to use.
The Attune Xenith cytometer can be a substantial-parameter spectral analyzer, offering both equally spectral unmixing and traditional flow capabilities for thorough and adaptable mobile Investigation.
The Attune CytPix Flow Cytometer permits users to visualise samples throughout acquisition, validate the precision of gating procedures, and assistance outcomes with dependable documentation for publication functions.
Below, we demonstrate that by gating procedures using graphic-centered quantitative parameters can distinguish interacting cells from coincident occasions to much more accurately examine interacting cells.